An Ammonia-Induced Calcium Phosphate Nanostructure: A Potential Assay for Studying Osteoporosis and Bone Metastasis

Osteoclastic resorption of bones plays a central role in both osteoporosis and bone metastasis. A reliable vitro assay that simulates osteoclastic vivo would significantly speed up the process developing effective therapeutic solutions for those diseases. Here, we reported development novel robust nanostructured calcium phosphate coating with unique functions on track-etched porous membrane by using an ammonia-induced mineralization (AiM) technique. The uniformly covers one side PET membrane, enabling testing resorption. pores allow mineral pins to grow inside, which, hand, enhances integration substrate and, other provides diffusion channels delivering drugs from lower chamber double-chamber cell culture system. applications processed were first demonstrated as drug screening device alendronate, widely used osteoporosis. It was confirmed delivery alendronate decreased number monocyte-differentiated osteoclasts To demonstrate application studying metastasis, delivered PC3 prostate cancer-conditioned medium differentiation monocytes into enhanced. This thus new platform activities assessing efficacy vitro.